CMM 465a/565a Fundamentals of Light Microscopy and Electronic Imaging

Instructor’s Name:  Dr. David Elliott

Course Description:  This is a lecture/hands-on course that teaches the essential principles and applications of light microscopy and electronic imaging.  By the end of the course you will be able to use and adjust a research microscope for various modes of light microscopy.

Credit hours:  3

Semester Offered:  Spring

This class will meet from 3-5 p.m. on Wednesdays in room 4137 of the Sarver Heart Center.  Labs will be TBA

Textbook:  Fundamentals of Light Microscopy and Electronic Imaging by Douglas B. Murphy, Wiley-Liss

Research papers will be assigned for specific topics.

The course grade will be based on quizzes, class participation and laboratory reports.

Week # - Description

  1. Light and Color
    Lab: Calibration of magnification with micrometer and reticule
  2. Koehler illumination, conjugate focal planes and resolution, objective lenses
    Lab: Diffraction and resolution
  3. Diffraction and image formation, and objective lens care
    Lab: Determination of hemoglobin concentration in RBC’s
  4. Phase contrast microscopy
    Lab: Measurements of birefringence and DIC examination of cells
  5. Polarized light microscopy, differential interference microscopy
    Lab: Molecular order determination using Polarized Light
  6. Fluorescence Microscopy, filters, dyes and illuminators
    Lab: Time-lapse movies using bright field
  7. Confocal Microscopy
  8. Alternative confocal designs
  9. Image Manipulation (Photoshop and NIH-Image/ImageJ)
  10. Convolution and Deconvolution
  11. Fluorescence filter sets and bleed through artifacts
  12. Visualizing protein-protein interactions
  13. Specimen preparation
  14. Digital Resolution
  15. Digital Image processing and printing
  16. Ethics