Gregory C. Rogers, PhD

Associate Professor, Cellular and Molecular Medicine
Associate Department Head, Faculty Development, Cellular and Molecular Medicine
Associate Professor, Molecular and Cellular Biology
Associate Professor, BIO5 Institute
Research Interests: 

I. Regulation of Centriole Duplication. Errors in chromosome segregation during cell division can result in the production of aneuploid daughter cells. This is particularly devastating during development, as aneuploidy is an underlying cause of miscarriage, birth defects, and cancers. During cell division, the accurate transmission of replicated chromosomes depends on the assembly of a bipolar spindle which is facilitated by the presence of centrioles, tiny organelles that help generate and organize spindle microtubules. Normally cells contain a single centriole pair, each duplicating only once prior to entering cell division. However, these mother centrioles have the capacity to assemble multiple daughters simultaneously. If cells assemble excess daughter centrioles (known as centriole amplification), then multipolar spindle assembly can ensue, leading to aneuploidy and increased risk for miscarriage/birth defects and cancer. In normal cells, what limits mother centrioles to assemble only a single daughter is unknown. It is known, however, that Polo-like kinase 4 (Plk4), the conserved master-initiator of centriole assembly, plays a key role in the duplication process. Therefore, a major goal of the Rogers lab is discovering how Plk4 works and how Plk4's activity is regulated.

II. Nuclear Organization During Interphase. A cell must organize its chromatin to accommodate the active transcription of genes and -- for those cells progressing through the cell cycle -- the replication of the cell's genome. One aspect of chromatin organization is its degree of compaction: while chromatin clearly undergoes extreme compaction during mitosis, the chromatin within interphase cells is also compacted but at a more moderate level. Importantly, interphase chromatin compaction is required for normal cellular function. In collaboration with Dr. Gio Bosco (Dartmouth), my lab is interested in understanding the regulation and physiological significance of interphase nuclear organization, particularly at the level of chromatin compaction.

Molecular Medicine Grad Program: 
Yes

Bibliography

2016

Galletta, B. J., C. J. Fagerstrom, T. A. Schoborg, T. A. McLamarrah, J. M. Ryniawec, D. W. Buster, K. C. Slep, G. C. Rogers, and N. M. Rusan, "A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4.", Nat Commun, vol. 7, pp. 12476, 2016. PMID: 27558293
Wang, M., R. B. Nagle, B. S. Knudsen, G. C. Rogers, and A. E. Cress, "A basal cell defect promotes budding of prostatic intraepithelial neoplasia.", J Cell Sci, 2016 Sep 8. PMID: 27609833

2015

Klebba, J. E., D. W. Buster, T. A. McLamarrah, N. M. Rusan, and G. C. Rogers, "Autoinhibition and relief mechanism for Polo-like kinase 4.", Proc Natl Acad Sci U S A, vol. 112, issue 7, pp. E657-66, 2015 Feb 17. PMCID: PMC4343128  PMID: 25646492
Wallace, H. A., J. E. Klebba, T. Kusch, G. C. Rogers, and G. Bosco, "Condensin II Regulates Interphase Chromatin Organization Through the Mrg-Binding Motif of Cap-H2.", G3 (Bethesda), vol. 5, issue 5, pp. 803-17, 2015 May. PMCID: PMC4426367  PMID: 25758823
Nguyen, H. Q., J. Nye, D. W. Buster, J. E. Klebba, G. C. Rogers, and G. Bosco, "Drosophila casein kinase I alpha regulates homolog pairing and genome organization by modulating condensin II subunit Cap-H2 levels.", PLoS Genet, vol. 11, issue 2, pp. e1005014, 2015. PMCID: PMC4344196  PMID: 25723539
Klebba, J. E., B. J. Galletta, J. Nye, K. M. Plevock, D. W. Buster, N. A. Hollingsworth, K. C. Slep, N. M. Rusan, and G. C. Rogers, "Two Polo-like kinase 4 binding domains in Asterless perform distinct roles in regulating kinase stability.", J Cell Biol, vol. 208, issue 4, pp. 401-14, 2015 Feb 16. PMCID: PMC4332252  PMID: 25688134
Bozler, J., H. Q. Nguyen, G. C. Rogers, and G. Bosco, "Condensins exert force on chromatin-nuclear envelope tethers to mediate nucleoplasmic reticulum formation in Drosophila melanogaster.", G3 (Bethesda), vol. 5, issue 3, pp. 341-52, 2015 Mar. PMCID: PMC4349088  PMID: 25552604

2014

Nye, J., D. W. Buster, and G. C. Rogers, "The use of cultured Drosophila cells for studying the microtubule cytoskeleton.", Methods Mol Biol, vol. 1136, pp. 81-101, 2014. PMID: 24633795
Skwarek, L. C., S. L. Windler, G. de Vreede, G. C. Rogers, and D. Bilder, "The F-box protein Slmb restricts the activity of aPKC to polarize epithelial cells.", Development, vol. 141, issue 15, pp. 2978-83, 2014 Aug. PMCID: PMC4197664  PMID: 25053431
Galletta, B. J., R. X. Guillen, C. J. Fagerstrom, C. W. Brownlee, D. A. Lerit, T. L. Megraw, G. C. Rogers, and N. M. Rusan, "Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.", Mol Biol Cell, vol. 25, issue 18, pp. 2682-94, 2014 Sep 15. PMCID: PMC4161505  PMID: 25031429

2013

Buster, D. W., S. G. Daniel, H. Q. Nguyen, S. L. Windler, L. C. Skwarek, M. Peterson, M. Roberts, J. H. Meserve, T. Hartl, J. E. Klebba, et al., "SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2.", J Cell Biol, vol. 201, issue 1, pp. 49-63, 2013 Apr 1. PMCID: PMC3613687  PMID: 23530065
Klebba, J. E., D. W. Buster, A. L. Nguyen, S. Swatkoski, M. Gucek, N. M. Rusan, and G. C. Rogers, "Polo-like kinase 4 autodestructs by generating its Slimb-binding phosphodegron.", Curr Biol, vol. 23, issue 22, pp. 2255-61, 2013 Nov 18. PMCID: PMC3844517  PMID: 24184097
Smith, H. F., M. A. Roberts, H. Q. Nguyen, M. Peterson, T. A. Hartl, X-J. Wang, J. E. Klebba, G. C. Rogers, and G. Bosco, "Maintenance of interphase chromosome compaction and homolog pairing in Drosophila is regulated by the condensin cap-h2 and its partner Mrg15.", Genetics, vol. 195, issue 1, pp. 127-46, 2013 Sep. PMCID: PMC3761296  PMID: 23821596
Brownlee, C. W., and G. C. Rogers, "Show me your license, please: deregulation of centriole duplication mechanisms that promote amplification.", Cell Mol Life Sci, vol. 70, issue 6, pp. 1021-34, 2013 Mar. PMID: 22892665

2012

Mennella, V., B. Keszthelyi, K. L. McDonald, B. Chhun, F. Kan, G. C. Rogers, B. Huang, and D. A. Agard, "Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization.", Nat Cell Biol, vol. 14, issue 11, pp. 1159-68, 2012 Nov. PMCID: PMC3767400  PMID: 23086239
Slevin, L. K., J. Nye, D. C. Pinkerton, D. W. Buster, G. C. Rogers, and K. C. Slep, "The structure of the plk4 cryptic polo box reveals two tandem polo boxes required for centriole duplication.", Structure, vol. 20, issue 11, pp. 1905-17, 2012 Nov 7. PMCID: PMC3496063  PMID: 23000383
Roberts, D. M., M. I. Pronobis, K. M. Alexandre, G. C. Rogers, J. S. Poulton, D. E. Schneider, K-C. Jung, D. J. McKay, and M. Peifer, "Defining components of the ß-catenin destruction complex and exploring its regulation and mechanisms of action during development.", PLoS One, vol. 7, issue 2, pp. e31284, 2012. PMCID: PMC3281067  PMID: 22359584

2011

Brownlee, C. W., J. E. Klebba, D. W. Buster, and G. C. Rogers, "The Protein Phosphatase 2A regulatory subunit Twins stabilizes Plk4 to induce centriole amplification.", J Cell Biol, vol. 195, issue 2, pp. 231-43, 2011 Oct 17. PMCID: PMC3198173  PMID: 21987638

2010

Buster, D. W., J. Nye, J. E. Klebba, and G. C. Rogers, "Preparation of Drosophila S2 cells for light microscopy.", J Vis Exp, issue 40, 2010. PMCID: PMC3153891  PMID: 20543772
Rogers, G. C., "Spindle assembly: more than just microtubules.", Curr Biol, vol. 20, issue 8, pp. R364-6, 2010 Apr 27. PMID: 21749958
Taylor, S. M., K. R. Nevis, H. L. Park, G. C. Rogers, S. L. Rogers, J. G. Cook, and V. L. Bautch, "Angiogenic factor signaling regulates centrosome duplication in endothelial cells of developing blood vessels.", Blood, vol. 116, issue 16, pp. 3108-17, 2010 Oct 21. PMCID: PMC2974614  PMID: 20664058

2009

Rusan, N. M., and G. C. Rogers, "Centrosome function: sometimes less is more.", Traffic, vol. 10, issue 5, pp. 472-81, 2009 May. PMID: 19192251
Rogers, G. C., N. M. Rusan, D. M. Roberts, M. Peifer, and S. L. Rogers, "The SCF Slimb ubiquitin ligase regulates Plk4/Sak levels to block centriole reduplication.", J Cell Biol, vol. 184, issue 2, pp. 225-39, 2009 Jan 26. PMCID: PMC2654306  PMID: 19171756
Rath, U., G. C. Rogers, D. Tan, M. Ana Gomez-Ferreria, D. W. Buster, H. J. Sosa, and D. J. Sharp, "The Drosophila kinesin-13, KLP59D, impacts Pacman- and Flux-based chromosome movement.", Mol Biol Cell, vol. 20, issue 22, pp. 4696-705, 2009 Nov. PMCID: PMC2777100  PMID: 19793918

2008

Hall, J. R., H. O. Lee, B. D. Bunker, E. S. Dorn, G. C. Rogers, R. J. Duronio, and J. Gowen Cook, "Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damage.", J Biol Chem, vol. 283, issue 37, pp. 25356-63, 2008 Sep 12. PMCID: PMC2533066  PMID: 18617514
Rogers, G. C., N. M. Rusan, M. Peifer, and S. L. Rogers, "A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells.", Mol Biol Cell, vol. 19, issue 7, pp. 3163-78, 2008 Jul. PMCID: PMC2441692  PMID: 18463166
Rogers, S. L., and G. C. Rogers, "Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy.", Nat Protoc, vol. 3, issue 4, pp. 606-11, 2008. PMID: 18388942

2007

Zhang, D., G. C. Rogers, D. W. Buster, and D. J. Sharp, "Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes.", J Cell Biol, vol. 177, issue 2, pp. 231-42, 2007 Apr 23. PMCID: PMC2064132  PMID: 17452528
Kim, H., S-C. Ling, G. C. Rogers, C. Kural, P. R. Selvin, S. L. Rogers, and V. I. Gelfand, "Microtubule binding by dynactin is required for microtubule organization but not cargo transport.", J Cell Biol, vol. 176, issue 5, pp. 641-51, 2007 Feb 26. PMCID: PMC2064022  PMID: 17325206

2005

Rogers, G. C., S. L. Rogers, and D. J. Sharp, "Spindle microtubules in flux.", J Cell Sci, vol. 118, issue Pt 6, pp. 1105-16, 2005 Mar 15. PMID: 15764594
Mennella, V., G. C. Rogers, S. L. Rogers, D. W. Buster, R. D. Vale, and D. J. Sharp, "Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase.", Nat Cell Biol, vol. 7, issue 3, pp. 235-45, 2005 Mar. PMID: 15723056

2004

Sharp, D. J., and G. C. Rogers, "A Kin I-dependent Pacman-flux mechanism for anaphase A.", Cell Cycle, vol. 3, issue 6, pp. 707-10, 2004 Jun. PMID: 15153812
Kwon, M., S. Morales-Mulia, I. Brust-Mascher, G. C. Rogers, D. J. Sharp, and J. M. Scholey, "The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility.", Mol Biol Cell, vol. 15, issue 1, pp. 219-33, 2004 Jan. PMCID: PMC307542  PMID: 14528012
Rogers, G. C., S. L. Rogers, T. A. Schwimmer, S. C. Ems-McClung, C. E. Walczak, R. D. Vale, J. M. Scholey, and D. J. Sharp, "Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase.", Nature, vol. 427, issue 6972, pp. 364-70, 2004 Jan 22. PMID: 14681690

2002

Rogers, S. L., G. C. Rogers, D. J. Sharp, and R. D. Vale, "Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle.", J Cell Biol, vol. 158, issue 5, pp. 873-84, 2002 Sep 2. PMCID: PMC2173155  PMID: 12213835

2001

Scholey, J. M., G. C. Rogers, and D. J. Sharp, "Mitosis, microtubules, and the matrix.", J Cell Biol, vol. 154, issue 2, pp. 261-6, 2001 Jul 23. PMCID: PMC2150769  PMID: 11470815

2000

Sharp, D. J., G. C. Rogers, and J. M. Scholey, "Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos.", Nat Cell Biol, vol. 2, issue 12, pp. 922-30, 2000 Dec. PMID: 11146657
Chui, K. K., G. C. Rogers, A. M. Kashina, K. P. Wedaman, D. J. Sharp, D. T. Nguyen, F. Wilt, and J. M. Scholey, "Roles of two homotetrameric kinesins in sea urchin embryonic cell division.", J Biol Chem, vol. 275, issue 48, pp. 38005-11, 2000 Dec 1. PMID: 11006281
Sharp, D. J., G. C. Rogers, and J. M. Scholey, "Microtubule motors in mitosis.", Nature, vol. 407, issue 6800, pp. 41-7, 2000 Sep 7. PMID: 10993066
Rogers, G. C., K. K. Chui, E. W. Lee, K. P. Wedaman, D. J. Sharp, G. Holland, R. L. Morris, and J. M. Scholey, "A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division.", J Cell Biol, vol. 150, issue 3, pp. 499-512, 2000 Aug 7. PMCID: PMC2175202  PMID: 10931863
Sharp, D. J., G. C. Rogers, and J. M. Scholey, "Roles of motor proteins in building microtubule-based structures: a basic principle of cellular design.", Biochim Biophys Acta, vol. 1496, issue 1, pp. 128-41, 2000 Mar 17. PMID: 10722882
Sharp, D. J., H. M. Brown, M. Kwon, G. C. Rogers, G. Holland, and J. M. Scholey, "Functional coordination of three mitotic motors in Drosophila embryos.", Mol Biol Cell, vol. 11, issue 1, pp. 241-53, 2000 Jan. PMCID: PMC14771  PMID: 10637305

1999

Rogers, G. C., C. L. Hart, K. P. Wedaman, and J. M. Scholey, "Identification of kinesin-C, a calmodulin-binding carboxy-terminal kinesin in animal (Strongylocentrotus purpuratus) cells.", J Mol Biol, vol. 294, issue 1, pp. 1-8, 1999 Nov 19. PMID: 10556023

1997

Kashina, A.S.., G.C.. Rogers, and J.M.. Scholey, "The bimC family of kinesins: essential bipolar mitotic motors driving centrosome separation.", Biochim Biophys Acta, vol. 1357, issue 3, pp. 257-271, 1997.