The ability of the epithelial cells to serve as a selective barrier to pathogens while absorbing nutrients is fundamental to their function. Tight junctions and associated polarity complexes are critical for selective permeability and maintenance of epithelial polarity. Our investigations of epithelial development and polarity focus on membrane trafficking to the junctions and apical membrane.
Jean Wilson, PhD
Professor, Cellular and Molecular Medicine
Professor, BIO5 Institute
Director, Willed Body Program
Molecular Medicine Grad Program:
Blum, I. R., C. Behling-Hess, M. Padilla-Rodriguez, S. Momtaz, C. Cox, and J. M. Wilson, "Rab22a regulates the establishment of epithelial polarity.", Small GTPases, pp. 1-12, 2020 Apr 17. PMID: 32281471
Jamwal, D. R., D. Laubitz, C. A. Harrison, V. Figliuolo da Paz, C. M. Cox, R. Wong, M. Midura-Kiela, M. A. Gurney, D. G. Besselsen, P. Setty, et al., "Intestinal Epithelial Expression of MHCII Determines Severity of Chemical, T-Cell-Induced, and Infectious Colitis in Mice.", Gastroenterology, vol. 159, issue 4, pp. 1342-1356.e6, 2020 10. PMID: 32589883
Engevik, A. C., I. Kaji, M. M. Postema, J. J. Faust, A. R. Meyer, J. A. Williams, G. N. Fitz, M. J. Tyska, J. M. Wilson, and J. R. Goldenring, "Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes.", J Cell Biol, vol. 218, issue 11, pp. 3647-3662, 2019 11 04. PMCID: PMC6829668 PMID: 31562230
Johnson, D. L., J. Wayt, J. M. Wilson, and J. G. Donaldson, "Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking.", J Cell Sci, 2017 Jun 05. PMID: 28584192
Parker, S. S., C. Cox, and J. M. Wilson, "Rabs set the stage for polarity.", Small GTPases, pp. 1-14, 2017 Jan 26. PMID: 28125340
Cox, C.M., E.K. Mandell, L. Stewart, R. Lu, D.L. Johnson, S.D. McCarter, A. Tavares, R. Runyan, S. Ghosh, and J.M. Wilson, Endosomal regulation of contact inhibition through the AMOT:YAP pathway., 2015.
Lu, R., D..L.Johnson, L. Stewart, K. Waite, and J. M. Wilson, Rab14 regulates epithelial lumen morphogenesis through regulation of claudin-2 trafficking. Molec Biol Cell 25:1744-54, 2014.
Parker, S. S., E. K. Mandell, S. M. Hapak, I. Y. Maskaykina, Y. Kusne, J-Y. Kim, J. K. Moy, P. A. St John, J. M. Wilson, K. M. Gothard, et al., "Competing molecular interactions of aPKC isoforms regulate neuronal polarity.", Proc Natl Acad Sci U S A, vol. 110, issue 35, pp. 14450-5, 2013 Aug 27. PMCID: PMC3761571 PMID: 23940317
McCarter, S., D. Johnson, KN. Kitt, C. Donohue, AE. Adams, and JM. Wilson, Regulation of tight junction assembly and epithelial polarity by a resident protein of apical endosomes. Traffic 11:856-866, 2010.
Kitt, KN., D. Hernandez-Deviez, S. McCarter, E. Spiliotis, J.E. Casanova, and J.M. Wilson, The small GTPase Rab 14 regulates apical targeting. Traffic 9:1218-1231, 2008.
Hernández-Deviez, D., A. Mackay-Sim, and J. M. Wilson, "A Role for ARF6 and ARNO in the regulation of endosomal dynamics in neurons.", Traffic, vol. 8, issue 12, pp. 1750-64, 2007 Dec. PMID: 17897316
Hernández-Deviez, D. J., J. E. Casanova, and J. M. Wilson, "Regulation of dendritic development by the ARF exchange factor ARNO.", Nat Neurosci, vol. 5, issue 7, pp. 623-4, 2002 Jul. PMID: 12032543
Gokay, K. E., R. S. Young, and J. M. Wilson, "Cytoplasmic signals mediate apical early endosomal targeting of endotubin in MDCK cells.", Traffic, vol. 2, issue 7, pp. 487-500, 2001 Jul. PMID: 11422942
Gokay, K. E., and J. M. Wilson, "Targeting of an apical endosomal protein to endosomes in Madin-Darby canine kidney cells requires two sorting motifs.", Traffic, vol. 1, issue 4, pp. 354-65, 2000 Apr. PMID: 11208120
Garver, W. S., R. A. Heidenreich, R. P. Erickson, M. A. Thomas, and J. M. Wilson, "Localization of the murine Niemann-Pick C1 protein to two distinct intracellular compartments.", J Lipid Res, vol. 41, issue 5, pp. 673-87, 2000 May. PMID: 10787428
Wilson, J. M., M. de Hoop, N. Zorzi, B. H. Toh, C. G. Dotti, and R. G. Parton, "EEA1, a tethering protein of the early sorting endosome, shows a polarized distribution in hippocampal neurons, epithelial cells, and fibroblasts.", Mol Biol Cell, vol. 11, issue 8, pp. 2657-71, 2000 Aug. PMCID: PMC14947 PMID: 10930461
Falk, T., W. S. Garver, R. P. Erickson, J. M. Wilson, and A. J. Yool, "Expression of Niemann-Pick type C transcript in rodent cerebellum in vivo and in vitro.", Brain Res, vol. 839, issue 1, pp. 49-57, 1999 Aug 21. PMID: 10482798
Garver, W. S., R. P. Erickson, J. M. Wilson, T. L. Colton, G. S. Hossain, M. A. Kozloski, and R. A. Heidenreich, "Altered expression of caveolin-1 and increased cholesterol in detergent insoluble membrane fractions from liver in mice with Niemann-Pick disease type C.", Biochim Biophys Acta, vol. 1361, issue 3, pp. 272-80, 1997 Oct 24. PMID: 9375801
Speelman, B. A., K. Allen, T. L. Grounds, M. R. Neutra, T. Kirchhausen, and J. M. Wilson, "Molecular characterization of an apical early endosomal glycoprotein from developing rat intestinal epithelial cells.", J Biol Chem, vol. 270, issue 4, pp. 1583-8, 1995 Jan 27. PMID: 7829488
Wilson, J. M., J. A. Whitney, and M. R. Neutra, "Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum.", J Cell Sci, vol. 100 ( Pt 1), pp. 133-43, 1991 Sep. PMID: 1795021
Wilson, J. M., N. Fasel, and J. P. Kraehenbuhl, "Polarity of endogenous and exogenous glycosyl-phosphatidylinositol-anchored membrane proteins in Madin-Darby canine kidney cells.", J Cell Sci, vol. 96 ( Pt 1), pp. 143-9, 1990 May. PMID: 1973690
Amerongen, H. M., J. A. Mack, J. M. Wilson, and M. R. Neutra, "Membrane domains of intestinal epithelial cells: distribution of Na+,K+-ATPase and the membrane skeleton in adult rat intestine during fetal development and after epithelial isolation.", J Cell Biol, vol. 109, issue 5, pp. 2129-38, 1989 Nov. PMCID: PMC2115838 PMID: 2553743