Reference
Teal, Emma, et al. “Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids”. Methods Mol Biol, vol. 1817, 2018, pp. 145-5, https://doi.org/10.1007/978-1-4939-8600-2_15.
Abstract
Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.