Reference
Hill, Samantha M, et al. “Optimized in Vitro Three-Dimensional Invasion Assay for Quantifying a Wide Range of Cancer Cell Invasive Behavior”. STAR Protoc, vol. 3, no. 3, Sept. 2022, p. 101516, https://doi.org/10.1016/j.xpro.2022.101516.
Abstract
We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).