Reference
Pond, Kelvin W, and Ellis, Nathan A. “Quantification of Double-Strand Breaks in Mammalian Cells Using Pulsed-Field Gel Electrophoresis”. Methods Mol Biol, vol. 1999, May 2019, pp. 75-85, https://doi.org/10.1007/978-1-4939-9500-4_4.
Abstract
The double-strand break (DSB) is the most cytotoxic type of DNA damage and measurement of DSBs in cells is essential to understand their induction and repair. Pulsed-field gel electrophoresis (PFGE) allows for quantitative measurement of DSBs in a cell population generated by DNA damaging agents. PFGE has the capacity to separate DNA molecules from several hundred base pairs to over six million base pairs. In the method described here, molecules from five hundred thousand to three million base pairs are consolidated into a single band on the gel that is readily analyzed.